NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 (PerkinElmer)
New & Improved Library Preparation Kit for Your RNA Sequencing Needs
The NEXTFLEX® Rapid Directional RNA-Seq kit 2.0 is designed to prepare directional, strand specific RNA libraries for sequencing using Illumina® sequencers. The kit streamlines the sample prep procedure required for single, paired-end, and multiplexed sequencing of RNA. The workflow is simplified by using master mixed reagents and magnetic bead-based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. In addition, the availability of up to 384 unique barcode adapters upon request and gel-free size selection allows for high-throughput, multiplexed sequencing. The procedure is ideal for insert sizes of >150 bp. Directionality is retained by adding dUTP during the second strand synthesis step as only the cDNA strand is sequenced.
HIGHLIGHTS
- Reliable: High coverage uniformity with low duplication rate
- Convenient: Optimized for use with 5 ng – 5 µg total RNA with reverse transcriptase and cleanup/size selection beads included
- Streamlined: Simple protocol validated with NEXTFLEX® poly(A) beads 2.0 and NEXTFLEX® RiboNaut™ rRNA depletion kit (human, mouse, rat)
- Flexible: Designed to work with NEXTFLEX® RNA-Seq 2.0 Unique Dual Index Barcodes that allow a wide range of multiplexing (2 up to 384 samples in one run)
- Efficient: Automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations
AUTOMATED RNA-SEQ WORKFLOW
RELATED PRODUCT - NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes
- For multiplexing sequencing libraries compatible with both paired-end and single-read Illumina® sequencing
- Every lot is functionally validated and tested for index purity by sequencing
- 96-well format with 2 reactions per barcode
- Up to 1,536 adapters available - users can multiplex anywhere between two to 1,536 libraries in a single sequencing run
RESOURCES
- Manual (.pdf)
- Specifications (.pdf)
- Sequences
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