NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0


NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 (PerkinElmer)


New & Improved Library Preparation Kit for Your RNA Sequencing Needs 

The NEXTFLEX® Rapid Directional RNA-Seq kit 2.0 is designed to prepare directional, strand specific RNA libraries for sequencing using Illumina® sequencers. The kit streamlines the sample prep procedure required for single, paired-end, and multiplexed sequencing of RNA. The workflow is simplified by using master mixed reagents and magnetic bead-based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. In addition, the availability of up to 384 unique barcode adapters upon request and gel-free size selection allows for high-throughput, multiplexed sequencing. The procedure is ideal for insert sizes of >150 bp. Directionality is retained by adding dUTP during the second strand synthesis step as only the cDNA strand is sequenced.




RELATED PRODUCT - NEXTFLEX® RNA-Seq 2.0 Unique Dual Index barcodes

  • For multiplexing sequencing libraries compatible with both paired-end and single-read Illumina® sequencing
  • Every lot is functionally validated and tested for index purity by sequencing
  • 96-well format with 2 reactions per barcode
  • Up to 1,536 adapters available - users can multiplex anywhere between two to 1,536 libraries in a single sequencing run



  • Manual (.pdf)
  • Specifications (.pdf)
  • Sequences  
    • Sequences of NEXTFLEX® RNA-seq 2.0 Unique Dual-Indexed Barcode (set of 384) Indexes – Excel / PDF
    • Sequences of NEXTFLEX® RNA-seq 2.0 Unique Dual-Indexed Barcode (set of 1,536) Indexes – Excel


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